Publications

2021

Feyen DAM, Perea-Gil I, Maas RGC, Harakalova M, Gavidia AA, Arthur Ataam J, Wu T-H, Vink A, Pei J, Suurmeijer AJ, Rijdt W, Vu M, Amatya PL, Prado M, Zhang Y, Sluijter JPG, Sallam K, Asselbergs FW, Mercola M, Karakikes I. The Unfolded Protein Response as a Compensatory Mechanism and Potential Therapeutic Target in PLN R14del Cardiomyopathy. Circulation. 2021;in press.

Stillitano F, Nour R, Zhang L, Fargnoli A, Hansen, A, Fava M, Yin X, Theofilatos K, Ceholski D, Kohlbrenner E, Jeong D, Wills L, Dave J, Nonnenmacher M, Haghighi K,  Costa KD, Turnbull IC, Mayr M, Cai C, Kranias EG, Akar FG, Hajjar RJ. Gene editing reverses arrhythmia susceptibility in humanized PLN-R14del mice: modeling a European cardiomyopathy with global impact (In Revision).

Hnatiuk AP, Briganti F, Staudt DW, Mercola M. Human iPSC modeling of heart disease for drug development. Cell Chem Biol. 2021;28(3):271-82.

Maas RGC, Lee S, Harakalova M, Snijders Blok CJB, Goodyer WR, Hjortnaes J, Doevendans PAFM, Van Laake LW, van der Velden J, Asselbergs FW, Wu JC, Sluijter JPG, Wu SM, Buikema JW. Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes. STAR Protoc. 2021 Feb 9;2(1):100334.

Pei J, Schuldt M, Nagyova E, Gu Z, el Bouhaddani S, Yiangou L, Jansen M, Calis JJA, Dorsch LM, Blok CS, van den Dungen NAM, Lansu N, Boukens BJ, Efimov IR, Michels M, Verhaar MC, de Weger R, Vink A, van Steenbeek FG, Baas AF, Davis RP, Uh HW, Kuster DWD, Cheng C, Mokry M, van der Velden J, Asselbergs FW, Harakalova M. Multi-omics integration identifies key upstream regulators of pathomechanisms in hypertrophic cardiomyopathy due to truncating MYBPC3 mutations. Clinical epigenetics, 2021, vol. 13, no. 1, 61. https://doi.org/10.1186/s13148-021-01043-3

Schuldt M, Pei J, Harakalova M, Dorsch LM, Schlossarek S, Mokry M, Knol JC, Pham TV, Schelfhorst T, Piersma SR, Remedios CD, Dalinghaus M, Michels M, Asselbergs FW, Moutin M, Carrier L, Jimenez CR, van der Velden J, Kuster DWD. Proteomic and Functional Studies Reveal Detyrosinated Tubulin as Treatment Target in Sarcomere Mutation-Induced Hypertrophic Cardiomyopathy. Circ Heart Fail. 2021 Jan;14(1):e007022. doi: 10.1161/CIRCHEARTFAILURE.120.007022. Epub 2021 Jan 12.

van Driel BO, Schuldt M, Algül S, Levin E, Güclü A, Germans T, Rossum ACV, Pei J, Harakalova M, Baas A, Jans JJM, van der Velden J. Metabolomics in Severe Aortic Stenosis Reveals Intermediates of Nitric Oxide Synthesis as Most Distinctive Markers. Int J Mol Sci. 2021 Mar 30;22(7):3569. doi: 10.3390/ijms22073569. PMID: 33808189; PMCID: PMC8037707

Gu Z, El Bouhaddani S, Pei J, Houwing-Duistermaat J, Uh HW. Statistical integration of two omics datasets using GO2PLS. BMC Bioinformatics. 2021 Mar 18;22(1):131. doi: 10.1186/s12859-021-03958-3. PMID: 33736604; PMCID: PMC7977326.

Peper J, Kownatzki-Danger D, Weninger G, Seibertz F, Pronto JRD, Sutanto H, Pacheu-Grau D, Hindmarsh R, Brandenburg S, Kohl T, Hasenfuss G, Gotthardt M, Rog-Zielinska EA, Wollnik B, Rehling P, Urlaub H, Wegener J, Heijman J, Voigt N, Cyganek L, Lenz C, Lehnart SE. Caveolin3 Stabilizes McT1-Mediated Lactate/Proton Transport in Cardiomyocytes. Circ Res. 2021 Mar 19;128(6):e102-e120.

Feyen DAM*, Perea-Gil I*, Maas RGC, Harakalova M, Gavidia AA, Wu TH, Vink A, Pei J, Suurmeijer A, Vu M, Amatya PL, Prado M, Zhang Y, Dunkenberger L, Sluijter JPG, Sallam K, Asselbergs FW, Mercola M, Karakikes I. The unfolded protein response as a compensatory mechanism and potential therapeutic target in PLN R14del cardiomyopathy. Circulation. 2021. Accepted. (*equal first author contribution)

Cheng J, Tsuda M, Okolotowicz K, Dwyer M, Bushway PJ, Colas AR, Lancman JJ, Schade D, Perea-Gil I, Bruyneel AAN, Lee J, Vadgama N, Quach J, McKeithan WL, Biechele TL, Wu JC, Moon RT, Si Dong PD, Karakikes I, Cashman JR, Mercola M. Small-molecule probe reveals a kinase cascade that links stress signaling to TCF/LEF and Wnt responsiveness. Cell Chem Biol. 2021; S2451-9456(21)00001-5.

Valanti EK, Dalakoura-Karagkouni K, Siasos G, Kardassis D, Eliopoulos AG, Sanoudou D. Advances in biological therapies for dyslipidemias and atherosclerosis. Metabolism. 2021 Mar;116:154461. doi: 10.1016/j.metabol.2020.154461

Munawar S, Turnbull IC*. Cardiac Tissue Engineering: Inclusion of Non-cardiomyocytes for Enhanced Features. Front Cell Dev Biol. 2021 May 25;9:653127. eCollection 2021. doi:10.3389/fcell.2021.653127.  *Senior and Corresponding author.

Turnbull IC*, Zhu W, Stillitano F, Chien C, Gaitas A*. A micromachined force sensing apparatus and method for human engineered cardiac tissue and induced pluripotent stem cell characterization. Sensors and Actuators A 331 (2021) 112874, https://doi.org/10.1016/j.sna.2021.112874. *Co-corresponding author.

2020

Arthur Ataam J, Amsallem M, Guihaire J, Haddad F, Lamrani L, Stephan F, Jaïs X, Humbert M, Mercier O, Fadel E. Preoperative C-reactive protein predicts early postoperative outcomes after pulmonary endarterectomy in patients with chronic thromboembolic pulmonary hypertension.J Thorac Cardiovasc Surg. 2020 Jan 3:S0022-5223(19)43490-9.

Amsallem M, Sweatt AJ, Arthur Ataam J, Guihaire J, Lecerf F, Lambert M, Ghigna MR, Ali MK, Mao Y, Fadel E, Rabinovitch M, de Jesus Perez V, Spiekerkoetter E, Mercier O, Haddad F, Zamanian RT.Targeted Proteomics of Right Heart Adaptation to Pulmonary Arterial Hypertension. Eur Respir J. 2020 Dec 17:2002428.

Feyen DAM, McKeithan WL, Bruyneel AAN, Spiering S, Hormann L, Ulmer B, Zhang H, Briganti F, Schweizer M, Hegyi B, Liao Z, Polonen RP, Ginsburg KS, Lam CK, Serrano R, Wahlquist C, Kreymerman A, Vu M, Amatya PL, Behrens CS, Ranjbarvaziri S, Maas RGC, Greenhaw M, Bernstein D, Wu JC, Bers DM, Eschenhagen T, Metallo CM, Mercola M. Metabolic Maturation Media Improve Physiological Function of Human iPSC-Derived Cardiomyocytes. Cell Rep. 2020;32(3):107925. PMCID: PMC7437654.

McKeithan WL, Feyen DAM, Bruyneel AAN, Okolotowicz KJ, Ryan DA, Sampson KJ, Potet F, Savchenko A, Gomez-Galeno J, Vu M, Serrano R, George AL, Jr., Kass RS, Cashman JR, Mercola M. Reengineering an Antiarrhythmic Drug Using Patient hiPSC Cardiomyocytes to Improve Therapeutic Potential and Reduce Toxicity. Cell Stem Cell. 2020;27(5):813-21 e6. PMCID: PMC7655512.

Paige SL, Galdos FX, Lee S, Chin ET, Ranjbarvaziri S, Feyen DAM, Darsha AK, Xu S, Ryan JA, Beck AL, Qureshi MY, Miao Y, Gu M, Bernstein D, Nelson TJ, Mercola M, Rabinovitch M, Ashley EA, Parikh VN, Wu SM. Patient-Specific Induced Pluripotent Stem Cells Implicate Intrinsic Impaired Contractility in Hypoplastic Left Heart Syndrome. Circulation. 2020;142(16):1605-8. PMCID: PMC7583658.

Pei J, Maas RGC, Nagyova E, Gho JMIH, Blok CS, van Adrichem I, Calis JJA, van Es R, Sepehrkhouy S, Feyen D, van den Dungen N, Lansu N, de Jonge N, den Ruijter HM, Huibers MMH, de Weger RA, van Laake LW, Verhaar MC, van Tintelen P, van Steenbeek FG, van Mil A, Buikema JW, Burgering B, Kararikes I, Mercola M, Doevendans PA, Sluijter J, Vink A, Cheng C, Mokry M, Asselbergs FW, Harakalova M. Transcriptional regulation profiling reveals disrupted lipid metabolism in failing hearts with a pathogenic phospholamban mutation. bioRxiv. 2020:2020.11.30.402792.

Kumar M, Haghighi K, Kranias EG, Sadayappan S. Phosphorylation of cardiac myosin binding protein-C contributes to calcium homeostasis. J Biol Chem. 2020 Aug 7; 295 (32): 11275-11291.

Anthony SR, Guarnieri A, Lanzillotta L, Gozdiff A, Green LC, O’Grady K, Helsley RN, Owens Iii AP, Tranter M. HuR expression in adipose tissue mediates energy expenditure and acute thermogenesis independent of UCP1 expression. Adipocyte. 2020 Dec;9(1):335-345.

Green LC, Anthony SR, Slone S, Neiman M, Xu L, Tranter M. Human Antigen R (HuR) modulates myofibroblast function and gene expression. Keystone Heart Failure Symposia, Keystone, CO, March 2020.

Green LC, Anthony SR, Slone S, Neiman M, Alam P, Tranter M. Human Antigen R (HuR) in the myofibroblasts is a critical regulator of pressure-overload induced cardiac remodeling. AHA BCVS virtual meeting, July 2020.

Menzel J, Kownatzki-Danger D, Tokar S, Ballone A, Unthan-Fechner K, Kilisch M, Lenz C, Urlaub H, Mori M, Ottmann C, Shattock MJ, Lehnart SE, Schwappach B. 14-3-3 binding creates a memory of kinase action by stabilizing the modified state of phospholamban. Sci Signal. 2020 Sep 1;13(647):eaaz1436.

Kownatzki-Danger D. Phospholamban – Identification of novel interaction partners. Doctoral Thesis; Göttingen Graduate School for Neurosciences, Biophysics and Molecular Biosciences, submitted February 2020.

Kumar M, Thangavel C, Becker RC, Sadayappan S. Monoclonal Antibody-Based Immunotherapy and Its Role in the Development of Cardiac Toxicity. Cancers (Basel). 2020 Dec 30;13(1).

Arif M, Nabavizadeh P, Song T, Desai D, Singh R, Bazrafshan S, Kumar M, Wang Y, Gilbert RJ, Dhandapany PS, Becker RC, Kranias EG, Sadayappan S. Genetic, clinical, molecular, and pathogenic aspects of the South Asian-specific polymorphic MYBPC3Δ25bp variant. Biophys Rev. 2020 Aug;12(4):1065-1084.

Zhou Y*, Ma J*, Zhu W. Efficacy and safety of direct oral anticoagulants versus warfarin in patients with atrial fibrillation across BMI categories: a systematic review and meta-analysis. American Journal of Cardiovascular Drugs. 2020 Feb;20(1):51-60.

Zhang J, Huang L, Shi X, Yang L, Hua F, Ma J, Zhu W, Liu X, Xuan R, Shen Y, Liu J. Metformin protects against myocardial ischemia-reperfusion injury and cell pyroptosis via AMPK/NLRP3 inflammasome pathway. Aging (Albany NY). 2020 Dec 15;12(23):24270.

Buikema JW, Lee S, Goodyer WR, Maas RG, Chirikian O, Li G, Miao Y, Paige SL, Lee D, Wu H, Paik DT, Rhee S, Tian L, Galdos FX, Puluca N, Beyersdorf B, Hu J, Beck A, Venkamatran S, Swami S, Wijnker P, Schuldt M, Dorsch LM, van Mil A, Red-Horse K, Wu JY, Geisen C, Hesse M, Serpooshan V, Jovinge S, Fleischmann BK, Doevendans PA, van der Velden J, Garcia KC, Wu JC, Sluijter JPG, Wu SM. Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes. Cell Stem Cell. 2020 Jul 2;27(1):50-63.e5.

Pei J*, Harakalova M*, Treibel TA, Lumbers RT, Boukens BJ, Efimov IR, van Dinter JT, González A, López B, El Azzouzi H, van den Dungen N, van Dijk CGM, Krebber MM, den Ruijter HM, Pasterkamp G, Duncker DJ, Nieuwenhuis EES, de Weger R, Huibers MM, Vink A, Moore JH, Moon JC, Verhaar MC, Kararigas G, Mokry M, Asselbergs FW, Cheng C. H3K27ac acetylome signatures reveal the epigenomic reorganization in remodeled non-failing human hearts. Clin Epigenetics. 2020 Jul 14;12(1):106.

Levitas A, Muhammad E, Zhang Y, Perea-Gil I, Serrano R, Diaz N, Arafat M, Gavidia AA, Kapiloff MS, Mercola M, Etzion Y, Parvari R, Karakikes I. A novel recessive mutation in SPEG causes early onset dilated cardiomyopathy. PLoS Genet. 2020; 16(9):e1009000.

LaRocca TJ, Seeger T, Prado M, Perea-Gil I, Neofytou E, Mecham BH, Ameen M, Chang ACY, Pandey G, Wu JC, Karakikes I. Pharmacological Silencing of MicroRNA-152 Prevents Pressure Overload-Induced Heart Failure. Circ Heart Fail. 2020; 13(3):e006298.

Perea-Gil I, Belbachir N, Gavidia AA, Arthur JA, Zhang Y, Vadgama ND, Oikonomopoulos A, Roura S, Wu JC, Bayes-Genis A, Karakikes I.. Activation of CaMKII Signaling Pathway Contributes to the Pathogenesis of Genetic Hypertrophic Cardiomyopathy. [Abstract, poster presentation in Basic Cardiovascular Science Scientific Sessions; Boston, MA; July 2020]

Camille C. Van Neste, Joshua Mayourian, Francesca Stillitano, Irene C. Turnbull, Kevin D. Costa. Investigating the role of extracellular environment on the pro-contractile effects of the mesenchymal stem cell secretome. The New York stem cell foundation (NYSCF) Conference October 20-21,2020, location: virtual. Poster session.

Vafiadaki E, Arvanitis DA, Eliopoulos AG, Kranias EG, Sanoudou D. The Cardioprotective PKA-Mediated Hsp20 Phosphorylation Modulates Protein Associations Regulating Cytoskeletal Dynamics. Int J Mol Sci. 2020 Dec 16;21(24):9572. doi: 10.3390/ijms21249572.

Zhang Y, Karakikes I. Translating genomic insights into cardiovascular medicines: Opportunities and challenges of CRISPR-Cas9. Trends Cardiovasc Med. 2020 Jun 27;. doi: 10.1016/j.tcm.2020.06.008. [Epub ahead of print] Review. PubMed PMID: 32603681; PubMed Central PMCID: PMC7765739.

2019

Antonios G, Kusch H, Henke C, Suhr M, Schwappach B, Wagner E, Wegener J, Kohl T, Nussbeck SY, Lehnart SE. Online workflow platform for advanced light microscopy. German Society for Biochemistry and Molecular Biology (BGM) 70th Mosbacher Kolloquium – “High-resolution imaging of cells and molecules”. Mosbach 2019 https://gbm-online.de/tagungskalender-details/id-70-mosbacher-kolloquium-high-resolution-imaging-of-cells-and-molecules.html

Arthur Ataam J, Bouajila S, Amsallem M, Alpert A, Martin BA, Kobayashi Y, David Furman D, Moneghetti KJ, Rosenberg-Hasson Y,Contrepois K, Davis MM, Dekker CL, Wu JC, Sayed N, Kuznetsova T, Karakikes I, Orr SSS, Maecker HT, Haddad F, Circulating Immune Profile Of Aging, A Translational Study On The Role Of Growth Differentiation Factor-15 And Monokine Induced By Interferon Gamma. [Abstract, poster presentation in American Heart Association Scientific Sessions; Philadelphia, PA; November 2019]

Arvanitis, DA and Sanoudou D. Cardio-oncology vs Onco-cardiology: Any common molecular mechanisms? [Abstract, oral presentation in 4th Cardio-oncology and Heart Failure meeting, Athens 2019]

Crocker, J.S., Vieira, D.C.O., Parks, K.G., O’Rourke, B., Dey, S.,  DeMazumder, D. Selective in vivo Modulation of Vagal-Muscarinic Signaling Prevents Sudden Cardiac Death in a Pressure Overload Model of Heart Failure. Circulation Research, vol. 125, issue Suppl_1(2019). (Scored: top 10 research). https://www.ahajournals.org/doi/10.1161/res.125.suppl_1.501

Vieira, D.C.O., Crocker, J.S., Desai, K., Sanagala, N., Wendelken, D., Parks, K.G., Liu, T., O’Rourke, B., Dey, S., DeMazumder, D.  Molecular, Cellular and Systemic Mechanism of Nonlinear Dynamic Patterns of Ventricular Repolarization and Spontaneous Arrhythmic Sudden Death in Non-ischemic Heart Failure. Circulation Research, vol. 125, issue Suppl_1(2019) (scored: top 10 research)  https://www.ahajournals.org/doi/10.1161/res.125.suppl_1.102

Gardner GT, Travers JG, Qian J, Liu G-S, Haghighi K, Robbins NJ, Jiang M, Li Y,  Fan G-C, Rubinstein J, Blaxall BC, Kranias EG: Phosphorylation of Hsp20 Promotes Fibrotic Remodeling and Heart Failure. JACC Basic Transl Sci. 2019 Apr 29;4(2):188-199. PMID: 31061921

Kownatzki-Danger D, Lenz C, Urlaub H, Lehnart SE. Analysis of native cardiac protein complexes by Complexome Profiling. Abstract, Göttingen Proteomics Forum, 2019. http://gpf.gwdg.de/GPF/GPF-events.html

Pei J, Juni R, Harakalova M, Duncker DJ, Asselbergs FW, Koolwijk P, Hinsbergh VV, Verhaar MC, Mokry M, Cheng C. Indoxyl Sulfate Stimulates Angiogenesis by Regulating Reactive Oxygen Species Production via CYP1B1. Toxins (Basel). 2019 Aug 2;11(8). pii: E454. doi: 10.3390/toxins11080454.

Hemerich D, Pei J, Harakalova M, van Setten J, Boymans S, Boukens BJ, Efimov IR, Michels M, van der Velden J, Vink A, Cheng C, van der Harst P, Moore JH, Mokry M, Tragante V, Asselbergs FW. Integrative Functional Annotation of 52 Genetic Loci Influencing Myocardial Mass Identifies Candidate Regulatory Variants and Target Genes. Circ Genom Precis Med. 2019 Feb;12(2):e002328. doi: 10.1161/CIRCGEN.118.002328.

Lee J, Termglinchan V, Diecke S, Itzhaki I, Lam CK, Garg P, Lau E, Greenhaw M, Seeger T, Wu H, Zhang JZ, Chen X, Perea-Gil I, Ameen M, Sallam K, Rhee JW, Churko JM, Chaudhary R, Chour T, Wang PJ, Snyder MP, Chang HY, Karakikes I, Wu JC. Activation of PDGF pathway links LMNA mutation to dilated cardiomyopathy. Nature. 2019; 572(7769):335-340.

Krishnan P, Moreno PR, Turnbull IC, Purushothaman M, Zafar U, Tarricone A, Singla S, Kini A, Sharma S, Narula J, Badimon JJ, Purushothaman KR. Incremental effects of diabetes mellitus and chronic kidney disease in medial arterial calcification: Synergistic pathways for peripheral artery disease progression. Vasc Med. 2019 Oct;24(5):383-394.PMID: 31090495.

Murphy JF, Mayourian J, Stillitano F, Munawar S, Broughton KM, Sussman MA, Hajjar RJ, Costa KD, Turnbull IC. Adult Human Cardiac Stem Cell-Supplementation Effectively Increases Contractile Function and Maturation in Human Engineered Cardiac Tissues. Stem Cell Research & Therapy, 2019 Dec 4;10(1):373.PMID: 31801634.

Salazar SI, Mayourian J, Chionuma H, Murphy J., Costa KD, Turnbull IC. Comparison of cell culturing conditions and extracellular vesicles preservation techniques to maximize cardioactive potency of the adult stem cell secretome. Extracellular Vesicles in Diagnostics and Therapeutics Symposium [Abstract, poster presentation in New York Academy of Sciences, February 19, 2019].

Stillitano F, Turnbull IC, Dave J, Hulot J, Hajjar RJ. Development of human engineered cardiac tissue (hECT)-based screening assay to explore cardiac contractile properties in response to pharmacological challenge with proarrhythmic drugs [Abstract, poster presentation in Translational Science, Meeting of the Association for Clinical and Translational Science; Washington, DC; March 5-8,2019].

Gaitas A*, Stillitano F, Turnbull I*. Establishing correlation of axial and vertical force of cardiac artificial tissue using a novel micromachined sensor. bioRxiv doi:https://doi.org/10.1101/2020.09.16-19, 2019]. .300665. This article is a preprint and has not been certified by peer review. *Corresponding authors.