Protocols and Standard Operating Procedures (SOPs)
Protocols and Standard Operating Procedures (SOPs)
Within CURE-PLaN the following Procedures and SOPs have been developed.
Non-cofidential documents can be downloaded from this page directly. Confidenial documents are just listed and members of CUREPLAN can find these documents in our online storage. If you are not a member of CUREPLAN, you can request these documents at the Consortium’s contact point.
Preclinical
- Adult Mouse Cardiac Myocyte – Kranias lab
- Blot overlay assay Sanoudou lab
- Calcium Uptake Procedure – Kranias lab
- CRISPR-HDR – KarakikesLab
- Differentiation iPSC-CM – KarakikesLab
- ECG and Arrhythmias – Kranias lab
- GSA Protocol DNA shipment – van Tintelen lab
- GST protein expression and purification – Sanoudou lab
- Immunofluorescence Cardiomyocytes – Kranias lab
- Immunofluorescence HEK – Sanoudou lab
- Immunostaining on fixed isolated Ventricular Cardiomyocytes – Kranias lab
- Infinium global screening array – van Tintelen lab
- Mouse isolation buffers – Kranias lab
- PLN-Western – Kranias lab
- Pull-down assay – Sanoudou lab
- ALMN Online Platform – Lehnart Lab
- Ratiometric Proximity Proteomic Analysis by APEX2 PLN NRCM Adenoviral Transfection – Lehnart Lab
- Immunofluorescence of iPSCs(-CMs) – Stillitano Lab
- hECT fabrication protocol – Costa Lab