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Protocols and SOPseelcosoeteman2019-11-16T20:05:24+00:00

Protocols and Standard Operating Procedures (SOPs)

Protocols and Standard Operating Procedures (SOPs)

Within CURE-PLaN the following Procedures and SOPs have been developed.

Non-cofidential documents can be downloaded from this page directly. Confidenial documents are just listed and members of CUREPLAN can find these documents in our online storage. If you are not a member of CUREPLAN, you can request these documents at the Consortium’s contact point.

Preclinical

    • Adult Mouse Cardiac Myocyte – Kranias lab
    • Blot overlay assay Sanoudou lab
    • Calcium Uptake Procedure – Kranias lab
    • CRISPR-HDR – KarakikesLab
    • Differentiation iPSC-CM – KarakikesLab
    • ECG and Arrhythmias – Kranias lab
    • GSA Protocol DNA shipment – van Tintelen lab
    • GST protein expression and purification – Sanoudou lab
    • Immunofluorescence Cardiomyocytes – Kranias lab
    • Immunofluorescence HEK – Sanoudou lab
    • Immunostaining on fixed isolated Ventricular Cardiomyocytes – Kranias lab
    • Infinium global screening array – van Tintelen lab
    • Mouse isolation buffers – Kranias lab
    • PLN-Western – Kranias lab
    • Pull-down assay – Sanoudou lab

Clinical

  • PLN_Clinical workup
    • PLN MRI protocol
    • PLN Echocardiography protocol
    • PLN REDCap data dictionary
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